Design a human growth hormone expression plasmid?
Design a human growth hormone expression plasmid with the aid of a diagram. Identify the different components of the plasmid and the host for the propagation of the plasmid. thts the ques i hv been asked could some one pleeeeeeeeeaaaasssse help me
Tagged with: different components • expression • Human Growth Hormone • plasmid • propagation
Filed under: Human Growth Hormone
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First, u’ve to isolate the mRNA of the growth hormone u want to express in the plasmid. The alternative way is, sequence the peptide hormone(assuming the hormone to be peptide hormone, because otherwise expression in plasmid makes little sense.) Then synthesize artificially a polynucleotide that will give the desired GH (Growth Hormone) when transcripted or translated.
If u use prokarytic host, u’ve to use clone the gene under a prokaryotic operon or regulatory system. Using eukaryotic host is sometimes complex.
Guess that helps.